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August 2015

Volume 1 Issue 2

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Research Article

Immunological Responses in the Liver, Kidney, and Spleen of Atlantic Halibut Infected with atypical Aeromonas salmonicida using cDNA microarrays

Kyoung C. Park*, Jane A. Osborne, Laura L. Brown, Stewart C. Johnson

We investigated the differential gene expression within Atlantic halibut (Hippoglossus hippoglossus) in response to atypical A. salmonicida infection using a 381 cDNA microarray. Atlantic halibut were infected with atypical A. salmonicida strain 0203 and held at 10-12oC. Liver, kidney and spleen tissues were collected at 21 days post infection (DPI), and total RNA was isolated for microarray analysis.

                                                                                                                                                                                     Download PDF Full text ________________________________________________________________________________________________________________

Research Article

Microarray Analysis in the Liver and Kidney of Atlantic halibut (Hippoglossus hippoglossus) following Immunostimulation with a Commercial Vaccine Against Vibrio (Listonella) anguillarum and Aeromonas salmonicida

Kyoung C. Park*, Jane A. Osborne, Laura L. Brown, Stewart C. Johnson

The response of Atlantic halibut (Hippoglossus hippoglossus) to immunostimulation with a commercial vaccine was investigated using an Atlantic halibut cDNA microarray. In this study fish were immunostimulated with the commercial injectable vaccine and held at 10-12oC. At 1, 2, 7, 14 days post-immunostimulation liver and kidney tissues were collected and mRNA was purified and used for microarray, quantitative real time-PCR (qRT-PCR) and reverse transcription-PCR (RT-PCR) analysis. Of the 381 genes on the microarray, we identified 83 different genes that were differentially expressed (≥2-fold change, p<0.05) in at least one of the samples: 44 in liver, 48 in kidney and 8 in both.

                                                                                                                                                                                     Download PDF Full text ________________________________________________________________________________________________________________

Research Article

The Interaction of Tritium with Some Types of Aquatic Plants

Lydia Bondareva, Margarita Chebotina, Mikail Subbotin

Tritium is the least toxic radionuclide. The main contribution into the total tritium content into ecosystems is made by technogenic tritium which is due to the operation of nuclear fuel cycle enterprises. The tritium content in the ecosystem of the River Yenisei is connected with its background values as well as with tritium entering the water ecosystem as a result of the operation of the Mining and Chemical Combine, MCC Rosatom. Presented here are the investigations of the possible transformation of tritium interacting with certain species of aqueous plants – submerged macrophyte Elodea canadensis and aqueous plant, floating on the surface of water reservoirs, Lemna minor.

                                                                                                                                                                                     Download PDF Full text

 

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