Volume 2 Issue 1
Simultaneous Real-Time Monitoring of Glucose and Cholesterol Levels in Fish Using Wireless Biosensor System
Haiyun Wu, Kyoko Hibi, Takeshi Oomori, Masataka Murata, Hitoshi Ohnuki, Hideaki Endo*
Fish health condition can be evaluated by monitoring some changes of blood indicators. For example, blood glucose levels are closely correlated with stress levels and blood cholesterol levels are closely correlated with immune system function in fish. We developed a wireless enzyme sensor system to simultaneously monitor blood glucose and cholesterol levels in fish using separate electrodes in real-time. The electrodes were constructed with Pt-Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. Glucose oxidase or a mixture of cholesterol oxidase and cholesterol esterase was immobilized on the working electrode using glutaraldehyde.
Initial Investigations of Cloves and Clove Oil Component as Water Mold Inhibitors
Sierra Hauff, Michael E. Barnes*
A need exists for less-problematic chemical control of fungal (water mold) infestations on fish eggs during hatchery incubation. This study examined the antifungal properties of cloves Syzygium aromaticum and eugenol, the main active ingredient in clove oil. In the first experiment, ground cloves significantly affected the timing of water mold infestation on sesame seeds subjected to fungal spores during static incubation in Petri dishes. Visible fungal growth was observed beginning at 48 hours in control dishes, while growth was not observed in any of the dishes containing ground cloves for the duration of the study.
Effect of Dietary Inclusion of Genetically Modified Maize (MON89034) on Marine Medaka Oryzias dancena
Youn Kyoung Kim, Hyo Sun Jung, Sung Hwoan Cho and Dong Soo Kim*
This study was designed to investigate changes in marine medaka (Oryzias dancena) fed for 30 weeks on experimental diets containing genetically modified (GM) maize (event name: MON89034), in terms of fish growth, egg development, histopathology, and PCR analysis. The total maize content was maintained at 21% in the four experimental diets (Con, GM7, GM14, and GM21), but the MON89034 maize content was adjusted to 0%, 7%, 14% and 21%, respectively. The fish survival rate did not differ among the experimental diets, and no significant differences were observed in the final mean weight, fertilization, hatching, and abnormalities among the experimental groups, and no histological effects were observed.